A Comparative Study to Assess Relationship of Placental Weight in Normal Pregnant Women and in Anemic Pregnant Women in Selected Hospital of Pune City

 

Ms Supriya Chinchpure

Lecturer, Sadhu Vaswani college of Nursing, 10/10-1, Koregaon Road, Pune 411001

*Corresponding Author Email: supriyachinchpure@gmail.com

ABSTRACT: 

Background: Nearly 600,000 women die each year as a result of complications of pregnancy and childbirth; most of these deaths could be prevented with attainable resources and skills (WHO 1996). In India, anemia affects an estimated 50 percent of the population. A key component of Safe Motherhood is the eradication of anemia during pregnancy.  Over the course of a pregnancy, maternal blood volume increases by approximately 50%, from 4 to 6 L, the plasma volume increases somewhat more than the total RBC volume, leading to a fall in the hemoglobin concentration and hematocrit values. This decrease is smaller in women who take supplemental iron, whereas the fall may be dramatic in women who do not take supplemental iron and have limited iron stores or are anemic upon becoming pregnant. The placenta is the physiological link between the mother and her unborn infant. Abnormal placental growth is associated with adverse pregnancy outcomes disproportionately heavy placenta, suggestive of placental hypertrophy, may indicate an adaptive response to an adverse intrauterine environment. Placental hypertrophy may occur in the presence of conditions such as maternal anemia, cigarette smoking and lower socio-economic status. Conversely, a disproportionately small placenta may indicate poor nutrient supply to the placenta, or hypoxia resulting in placental growth restriction and subsequently fetal growth restriction. When there is a limitation imposed on fetal growth velocity due to nutritional deficiencies, the placenta may undergo hypertrophy in an attempt to compensate. As well, placental hypertrophy may occur in response to fetal /placental hypoxemia. It is also possible that using the placental ratio as a marker, infants with possible in-utero growth disturbance can be identified so that their postnatal growth and future health status can be monitored. Thus there may be effect of maternal anemic condition on the placental growth in turn may affect the newborns growth and development Thus there may be effect of maternal anemic condition on the placental growth in turn may affect the newborns growth and development. .

 

Objectives:

      To compare the placental weight among normal & anemic pregnant women

      To correlate the placental weight with the baby’s weight

 

Design: A Correlational design was adopted for the study.

 

Setting: Government hospital of Pune city was selected.

 

Participants: 100 expectant mothers fulfilling the inclusion criteria were selected by non probability convenient sampling technique. Two groups: Group A consisted of 50 pregnant mother with Hb level above 10gm%. & Group B consisted of 50 pregnant women with Hb level below 10 gm %.

 

Methodology: The tool consisted of Semi structured interview and observation of placental parameters. The Tool was divided into Section I- Demographic data, Section II- Examination of placenta & Section III- Birth weight. All participants fulfilling the criteria were approached, consent was taken & data was collected over 24 hours according to the patients delivery timing. The average time taken to fill the questionnaire after delivery of the baby and placenta was almost 15-20 minutes.

 

Results: The findings revealed that, in comparison of placental weight between Group A (Normal cases) and Group B (Anemic cases) shows highly significant difference (P<0.001), similarly in comparison of birth weight in normal and anemic cases shows highly significant difference between Group A (Normal cases) and Group B (Anemic cases) (P<0.001).

In Correlation between placental weight and birth weight Group A (Normal cases) does not show significant difference (P >0.05) but shows a positive correlation in the scatter diagram. This means as the placental weight increases the birth weight also increases and vice-versa. Group B (Anemic cases) shows highly significant difference with a positive correlation (p >0.001) and in the scatter diagram also it shows positive correlation. This means as the placental weight increases the birth weight also increases and vice-versa

 

Conclusion: To conclude with the help of above findings this study provides us with evidence that increase or decrease in maternal Hb levels leads to changes in the placental weight. And due to alteration in placental weight it affects the birth weight.

 

KEY WORDS: Relationship, Placental weight, Normal pregnant women & anemic pregnant women.

 


INTRODUCTION:

As soon the news of pregnancy is splashed, the environment is completely changed, whatever the pains, sorrows all come to an end. The whole family is experiencing joy and is planning for welcome of the coming angel. Whenever a seed is sown it starts going through different stages like germination, development of various parts i.e. roots, stem, leaves etc. for this whole process adequate food, nutrients, water, sunlight and care is required. Similarly when a life starts growing inside the womb, it also goes through various developmental stages of life for this whole process it requires nutrients, care, identification of problems at earlier stage and providing prompt intervention to ensure safety of the mother and the baby. All pregnancies and deliveries are at risk. However, there are certain categories of pregnancies where the mother, the fetus or the neonate are in a state of increased jeopardy. About 20 to 30 percent pregnancies belong to this category. If we desire to improve obstetric results, this group must be identified and given extra care.

 

Maternal mortality continues to be a major health problem in the developing world. Nearly 600,000 women die each year as a result of complications of pregnancy and childbirth; most of these deaths could be prevented with attainable resources and skills (WHO 1996). In India, anemia affects an estimated 50 percent of the population. A key component of Safe Motherhood is the eradication of anemia during pregnancy. (1) 

 

Over the course of a pregnancy, maternal blood volume increases by approximately 50%, from 4 to 6 L, the plasma volume increases somewhat more than the total RBC volume, leading to a fall in the hemoglobin concentration and hematocrit values.

 

This decrease is smaller in women who take supplemental iron, whereas the fall may be dramatic in women who do not take supplemental iron and have limited iron stores or are anemic upon becoming pregnant which can effect on the placental growth in turn may affect the newborns growth and development.(2)

 

A cohort study on effect of maternal anemia on placental ratio was conducted by Farrukh Robina et al (2006) on one hundred pregnant women, 50 anemic and 50 having normal range of Hb. After delivery, weight of the new born and weight of placenta was recorded. The mean weight of the Newborn (±SD) in anemic group was 3.12 ± 0.45Kg and 3.18 + 0.35Kg in control group. The difference was statistically non-significant (P=0.445). The mean placental weight of anemic group was 0.58 ± 0.13Kg and that of control group was 0.52 ± 0.088Kg showing a significant increase (P<0.009). Feto-placental ratio was 0.193 + 0.035 (Mean ± SD) in anemic patients and 0.166 + 0.024 in control group. FP ratio of anemic patients was significantly higher than control group (P<0.001). The study shows a significantly large placental weight and a high fetoplacental ratio (3).

 

Another cross sectional study was done by Kidanto Hussein L, Ingrid Mogren, Gunilla Lindmark, Siriel Massawe, Lennarth Nystrom (2009) to assess risks of maternal anemic condition on preterm delivery and low birth weight (n= 174 anemic and 547 non-anemic women). The risk of preterm delivery increased significantly with the severity of anemia, with odds ratios of 1.4, 1.4 and 4.1 respectively for mild, moderate and severe anemia. The corresponding risks for LBW and VLBW were 1.2 and 1.7, 3.8 and 1.5, and 1.9 and 4.2 respectively. The risks of preterm delivery and LBW increased in proportion to the severity of maternal anemia. (4)

 

The placenta is the physiological link between the mother and her unborn infant.  Through the placenta nutrients (and possible the contaminants) are transferred from the mother to the infant, including life-sustaining oxygen.  There is exchange of waste products from the infant back to the mother for excretion also. The placenta is a fetal organ consisting of an umbilical cord, membranes (chorion and amnion), and parenchyma. Maternal or fetal disorders may have placental sequelae since the mother and fetus interface at this site. Conversely, primary placental abnormalities can affect both maternal and fetal health. Thus, examination of the placenta may yield information on the impact of maternal disorders on the fetus or the cause of preterm delivery, fetal growth restriction, or neuro developmental impairment. Placental examination is an essential component of the autopsy in cases of fetal or neonatal death and even has clinical significance. (Roberts J Drucilla).

 

Many studies have shown relationship of placental weight to birth weight, a longitudinal cross-sectional study was conducted by Asgharnia. M, Esmailpour. N, Poorghorban. M and Atrkar-Roshan. Z (2008) to determine placental weight and factors associated with low weight placentas (n=1088). The subjects were categorized in high (> 750 g), normal (330-750 g), and low placental weights (< 330 g). The mean and standard deviation of neonates' weights at birth and placental weights were 3214.28 ± 529 and 529.72 ± 113 g, respectively and showed statistically significant relationships between placental weight and birth weight, fetal distress, Apgar score, maternal diabetes, pre-eclampsia and approaches of deliveries (α = 0.05). (5)

 

Another study was conducted by Sanin Luz Helena et al (2001) to assess Relation between Birth Weight and Placenta Weight. The mean of birth weight was 3,369 g with a standard deviation (SD) of 445 g. Placenta weight had a mean of 537 g (SD: 96 g). The relation between the weight of the placenta and the birth weight was significant, and we found that for each gram increase in placenta weight, birth weight is increased by 1.98 g (SE = 0.25, p < 0.01) and this relation is not linear, since the quadratic term was significant. Placenta weight has a nonlinear relation to the birth weight and is an important predictor of birth weight. Together with the gestational age and the maternal age and size, it explains 32% of the variability of birth weight. Placenta weight can be a 'sentinel' indicator of nutritional and/or environmental problems (6)

 

Several studies show that abnormal placental growth is associated with adverse pregnancy outcomes disproportionately heavy placenta, suggestive of placental hypertrophy, may indicate an adaptive response to an adverse intrauterine environment. Placental hypertrophy may occur in the presence of conditions such as maternal anemia, cigarette smoking and lower socio-economic status. Conversely, a disproportionately small placenta may indicate poor nutrient supply to the placenta, or hypoxia resulting in placental growth restriction and subsequently fetal growth restriction. When there is a limitation imposed on fetal growth velocity due to nutritional deficiencies, the placenta may undergo hypertrophy in an attempt to compensate. As well, placental hypertrophy may occur in response to fetal /placental hypoxemia. (7)

 

It is also possible that using the placental ratio as a marker, infants with possible in-utero growth disturbance can be identified so that their postnatal growth and future health status can be monitored. Thus there may be effect of maternal anemic condition on the placental growth in turn may affect the newborns growth and development. Thus there is a felt need to assess any effect of the maternal anemic condition on the placenta through placental examination. And further correlate the changes of placental weight with the birth weight of the baby.

 

STATEMENT OF PROBLEM:

“A comparative study to assess relationship of placental weight in normal pregnant women & in anemic pregnant women in selected hospital of Pune

 

OBJECTIVES:

·         To assess placental weight in normal pregnant women.

·         To assess placental weight in anemic pregnant women

·         To compare the placental weight among normal & anemic pregnant women

·         To correlate the placental weight with the baby’s weight

 

HYPOTHESIS:  There is no significant effect of maternal anemic condition on placental weight and baby weight.

 

ASSUMPTIONS:

   The study assumes that-

Ø  Anemia during pregnancy has an effect on the placental weight.

Ø  Anemia during pregnancy has an effect on the baby’s weight.

Ø  Any change in the placental weight has an effect on the baby’s weight.

 

INCLUSION CRITERIA:

1.       Pregnant mothers above 18 yrs of age.

2.       Period of gestation should be 37 completed weeks.

3.       Pregnant mothers having Hb level above 10 gm%. (normal )

4.       Pregnant mothers having Hb level below 10 gm%. (anemia)

5.       Pregnant mothers who have registered.

 

EXCLUSION CRITERIA-

1.       Pregnant mothers having any medical problems like hypertension, Diabetes mellitus, tuberculosis, TORCH, threatened abortion, fever or infection, etc thorough out the pregnancy or have taken medications for treatment of any medical problems arising during pregnancy.

 

MATERIALS AND METHODS:

Research Approach:

Research approach is a systematic, objective method of discovery with empirical evidence and rigorous control. Research that explores the interrelationships among variables of interest without intervention on the part of the researcher is a correlation study. In this study the investigator intended to correlate placental weight and baby’s weight.

 

Research Design:

The research design selected for the present study was exploratory in nature, which aims at finding out the relationship of placental weight in normal pregnant women & anemic pregnant women and further correlate the placental weight with baby’s weight. Thus an exploratory correlational was found to be appropriate research design.

 

Variables:

Independent Variable: Maternal anemic condition i.e. normal/anemic.

Dependent Variable: Placental weight.

Demographic variables:  Age, education, occupation, income, type of family, dietary habits, gravida and parity.

 

Research Setting:

The study was conducted in a corporation hospital of Pune city

 

Population:

The target population consists of people or things that meet the designated set of criteria of interest to the researcher. The population of the present study comprises of all the pregnant mothers irrespective of gravidity, completing 37 weeks of gestation, who have registered themselves.

Accessible population is the aggregate of participants who confirm to designate criteria and are accessible as a pool of subjects for a study (Polit and Beck 2004). The accessible population for the study was all registered cases of pregnant mothers from selected Hospital. One group consists of pregnant mothers who have their Hb levels above 10gm% and do not have any medical history throughout the pregnancy. Another group consists of pregnant mothers who have their Hb levels below 10gm% and do not have any medical history.

 

Sample:

Total number of expectant mothers selected for study was 100.

Group A- Pregnant mother with Hb level above 10gm%. (50 samples)

Group B- Pregnant women with Hb level below 10 gm %. (50 samples)

 

Sampling Technique:

Non Probability Purposive Sampling technique is used for the present study.

 

Development of Tool:

The data-gathering tool was constructed by the investigator in the light of the literature reviewed & her experience in the clinical field to ensure the adequacy & validity of the content.

The tool consisted of Semi structured interview and observation which had 3 sections-

 

Section I- Demographic data

 

Section II- Examination of placenta 

·         General examination

·         Maternal surface

·         Fetal surface

·         Umbilical cord

 

Section III- Birth weight

 

Description of Tool:

Section I: Demographic data includes characteristics like Age, Trimester at registration, Gravida, Parity, Hemoglobin levels (Hb),   Type of family, Monthly Family Income, Educational Qualification, Occupation, Dietary Pattern

 

Section II:

·         General examination of placenta includes completeness, shape, consistency, weight, diameter & thickness.

·         Maternal surface examination included color, consistency, lobes & abnormalities like clots, infarcts and calcium deposition.

·         Fetal surface examination includes abnormalities like circumvallate placenta, amnion nodusum, metaplasia, amnionic nodules, malodor etc.

·         Umbilical cord examination includes abnormalities like insertion (central, eccentric, marginal, velamentous), cord length, diameter, presence of true knots, abnormalities like abnormal no of vessels, thromboses etc.

 

Section III: Baby’s Birth Weight

 

Data collection Procedure:

It was planned to interview the samples on admission in labour room with initiation of labor process & collect the demographic data & obstetric history. History was collected from patients, Hb levels and no of visits from registration card, other relevant information from the primary & secondary sources. After the delivery of baby & placenta, the section II & section III was observed. Standard instrument i.e. measuring tape, weighing scale was used to record the data. The placenta was weighed on the standard weighing scale. Every time before weighing the scale was calibrated to zero. With the measuring tape the diameter, length of the cord was also measured from the centre of the placenta till the clamp and remaining cord with the baby was measured.  Both the length of the cord was added to get the total length of the cord. Thickness of the cord was measured by using a needle of 1 ½ inch length which was inserted in the centre of the placenta and measurement was taken till the point where needle was stained. Then thorough examination of placenta is done to assess maternal surface, fetal surface, umbilical cord.  At the end baby’s birth weight was measured on the standard weighing scale. All the findings are recorded on the sheet of individual client at the end of observation.

 


 

Plan for Data Analysis:

The data analysis was planned to include descriptive and inferential analysis. Descriptive analysis would be used for analysis of the demographic data and section II & III. Inferential statistics would be used to correlate the changes in placental weight with increase or decrease in maternal hemoglobin levels and correlate the changes in newborn birth weight with increase or decrease in the placental weight.

 

RESULTS:

Findings of section I:

Section I consist of demographic data which included age, trimester at registration, gravida, parity, type of family, family Income, education and diet. The major findings revealed are as follows-

Majority (67%) of the mothers belong to age group of 18-23 yrs, among which 35% belongs to Group A (Normal subjects) and 32% belongs to Group B (Anemic subjects).45% of mothers have registered themselves in the second trimester, among which 17% belong to Group A (Normal subjects) and 28% belong to Group B (Anemic subjects). 62% mothers were primigravida in which 31% belong to Group A (Normal subjects) and 31% belong to group B (Anemic subjects). 74% mothers were Primipara in which 35% belong to Group A (Normal subjects and 39% belong to group B (Anemic cases). 54% mothers were residing in Nuclear family from whom 15% belong to Group A (Normal subjects) and 11% belong to group B (Anemic subjects). 38% mothers are educated upto secondary level from whom 20% belong to Group A (Normal subjects) and 18% belong to Group B (Anemic subjects). And 51% of mothers are Non-vegetarian from which 27% belong to Group A (Normal subjects) and 24% belong to Group B (Anemic subjects).

 

Findings of section II:

Section II is examination of placenta which consists of general examination, examination of maternal surface, examination of fetal surface and umbilical cord and the major findings revealed for the same are as follows:

Majorities (97%) of placenta were discoid shape from which 49% belong to Group A (Normal subjects) and 48% belong to Group B (anemic subjects). And 3% of placentas were oval shapes from which 1% belongs to Group A (Normal subjects) and 2% belong to Group B (anemic subjects).

The findings of the placental diameter, thickness & lobes showed that there was no significant difference between Group A (Normal subjects and Group B (Anemic subjects)

In maternal surface abnormalities majority (34%) infarcts were seen as in cases of Group B (Anemic subjects). Similarly in fetal surface abnormalities 10% of cases in Group B (Anemic subjects) were circumvallate placenta and 4% of cases with other abnormalities i.e. were present in group B (Anemic subjects) .

In umbilical cord insertion majority (35%) of cases were with central cord insertion from which 21% of cases belong to Group A (Normal subjects) and 14% of cases belong to Group B (Anemic subjects). And 35% of cases were with Marginal cord insertion from which 15% of subjects belong to Group A (Normal subjects) and 20% of subjects belong to Group B (Anemic subjects). And 2% of cases were with Velamentous cord insertion from Group B (Anemic subjects). Umbilical cord length & diameter did not show any significant difference between Group A (Normal subjects) and Group B (Anemic subjects).    

 

The comparison of placental weight shows highly significant difference between Group A (Normal cases) and Group B (Anemic cases). In group A the mean was 485.5 and standard deviation was 41.41, similarly in Group B the mean was 544.8 and standard deviation was 122.01. It showed highly significant difference (P value is <0.001). Thus it explains that as the hemoglobin levels altered changes in the placental weight was seen.

 

In comparison of birth weight in normal and anemic cases, highly significant difference between Group A (Normal cases) and Group B (Anemic cases) was seen. In Group A the mean was 2694.5 and standard deviation was 387.93, similarly in Group B the mean was 2035.5 and standard deviation was 150.18. It is evident by calculating the p value. The p value is 11.20.  It showed highly significant difference (P <0.0001) which explains that as the hemoglobin levels altered changes in the birth weight was seen.

 

Findings of Section III:

In Correlation between placental weight and birth weight Group A (Normal cases) does not show significant difference (P >0.05) but showed a positive correlation in the scatter diagram. This means as the placental weight increases the birth weight also increases and vice-versa. 

Group B (Anemic cases) shows highly significant difference (P >0.001) with a positive correlation. It is evident by calculating the R value. The R value is 0.63. Here so it showed highly significant difference and in the scatter diagram also it shows positive correlation. This means as the placental weight increases the birth weight also increases and vice-versa.

 

CONCLUSION:

According to the study objectives the following conclusions were made-

Ø  The comparison of placental weight shows highly significant difference between Group A (Normal cases) and Group B (Anemic cases) which explains that alteration in hemoglobin levels was seen with changes in the placental weight.

Ø  The comparison of birth weight in normal and anemic cases shows highly significant difference between Group A (Normal cases) and Group B (Anemic cases) which explains that alteration in hemoglobin levels was seen with changes in the birth weight.

Ø  In Correlation between placental weight and birth weight Group A (Normal cases) does not show significant difference (P >0.05) but shows a positive correlation in the scatter diagram which means as the placental weight increases the birth weight also increases and vice-versa. 

Ø  In Correlation between placental weight and birth weight Group B (Anemic cases) shows highly significant difference (P >0.001) with a positive correlation which means as the placental weight increases the birth weight also increases and vice-versa.

 

This study provides us with evidence that increase or decrease in maternal Hb levels leads to changes in the placental weight. And in turn placental weight it affects the birth weight.

 

Thus importance should be given to early registration of cases, with identification of risk factors or complications and prompt treatment. Preconceptional counseling is an important aspect for women in reproductive age group to prepare them for pregnancy and to ensure a safe motherhood.

 

RECOMMENDATIONS:

Based on the findings of the study the investigator wants to recommend further studies-

Ø  Replicated using larger population of mothers.

Ø  Study can be conducted only on primipara as the sample and findings may be observed.

Ø  A comparative study in different hospital setup i.e. government and private can be done.

Ø  A prospective study would be much more useful rather than retrospective.

Ø  A comparative study with Hb levels at different trimesters can be done.

 

REFERENCES:

1.        Benard BJ, Hakimi Mohammad, Pelletier David. An Analysis of Anemia and Pregnancy-Related Maternal Mortality. Journal of Nutrition (abstract). 2001 [cited 2010 January 1]; 131:604s-615s. Available from http:\\placenta researches\3\An Analysis of Anemia and Pregnancy-Related Maternal Mortality –Brabin et al_131 (2)604S -- Journal of Nutrition. htm.

2.        Yetter III F J. examination of placenta. American Academy of Family Physicians Journal (AAFP). 1998 (cited 2010 January 2]; 12: 1-12. Available from http:\\placenta researches\8\Examination of the Placenta - March 1, 1998 - American Academy of Family Physicians.htm.

3.        Farrukh Robina, Chaudhary Samina, Farrukh Kamal, Zaheera Saadia, Fahmeeda Naheed, Rehan N, Naila Yasmeen. Effect of maternal anemia on Placental ratio. Report of a conference in Pakistan. Oct - Dec 2006 2001[cited 2010 January 18]; 12(4):521-3.  Available from http:\\placenta researches\51\PakMediNet - Effect of maternal anemia on Placental ratio.mht.

4.        Kidanto LH, Mogren I, Lindmark G, Massawe S, Nystrom L. Risks for preterm delivery and low birth weight are independently increased by severity of maternal anaemia. South Africa Medical Journals. 2009 February [cited 2010 January 18]; 99(2):98-102. Available from http:\\placenta researches\41\ Risks for preterm delivery and low birth weight are independently increased by severity of maternal anaemia -- Kidanto et al_ 99(2):98-102-- South Africa Medical Journals.mht.

5.        Asgharnia M, Esmailpour N, Poorghorban M and Atrkar-Roshan. Placental weight and its association with maternal and neonatal characteristics. Tehran University of Medical Sciences Journals (abstract). 2008 [cited 2010 April 4]; 46(6): 467-472. Available from http:\\placenta researches\13\Abstract.mht.

6.        Sanin LH, Lopez SR, Olivares ET, Terrazas MC, Silva MR, Carrillo ML.  Relation between birth weight and placental weight. Neonatology, fetal and Neonatology Research Journal (abstract). 2001 [cited 2010 April 4]; 80(2):113-117. Available from http:\\G/Relation%20%between% 20Birth %20weight%20 and%20placenta%20weight.htm.

7.        Kesha Baptiste-Roberts, Carolyn Salafia M, Wanda Nicholson K, Duggan Anne, Wang Nae-Yuh, and Frederick Brancati L. Maternal risk factors for abnormal placental growth: The national collaborative perinatal project. BMC Pregnancy and Childbirth (abstract). 2008 [cited 2010 January 2]; 8:44. Available from http:\\placenta researches\6\Maternal risk factors for abnormal placental growth The national collaborative perinatal project.htm.

 

 

 

 

Received on 08.04.2014          Modified on 25.06.2014

Accepted on 10.07.2014          © A&V Publication all right reserved

Asian J. Nur. Edu. & Research 4(3): July- Sept., 2014; Page 307-313